KINETIC CHARACTERIZATION OF CATALYSIS BY THE CHEMOTAXIS PHOSPHATASE CHEZ: MODULATION OF ACTIVITY BY THE CHEYp SUBSTRATE*
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October 19, 2007 KINETIC CHARACTERIZATION OF CATALYSIS BY THE CHEMOTAXIS PHOSPHATASE CHEZ: MODULATION OF ACTIVITY BY THE CHEYp SUBSTRATE* Ruth E. Silversmith, Matthew D. Levin, Elmar Schilling, and Robert B. Bourret Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599-7290 and Department of Physiology, Development, and Neuroscience, University of Cambridge CB2 3EJ, United Kingdom Running title: Kinetics of CheZ phosphatase Address correspondence to: Ruth E. Silversmith, Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599-7290, Tel. 919 966-2679; Fax. 919-962-8103; E-mail: [email protected] Current address: Department of Hematology and Oncology, Regensburg University Medical Center, 93042 Germany
منابع مشابه
Kinetic characterization of catalysis by the chemotaxis phosphatase CheZ. Modulation of activity by the phosphorylated CheY substrate.
CheZ catalyzes the dephosphorylation of the response regulator CheY in the two-component regulatory system that mediates chemotaxis in Escherichia coli. CheZ is a homodimer with two active sites for dephosphorylation. To gain insight into cellular mechanisms for the precise regulation of intracellular phosphorylated CheY (CheYp) levels, we evaluated the kinetic properties of CheZ. The steady st...
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Two-component regulatory systems, in which phosphorylation controls the activity of a response regulator protein, provide signal transduction in bacteria. For example, the phosphorylated CheY response regulator (CheYp) controls swimming behavior. In Escherichia coli, the chemotaxis phosphatase CheZ stimulates the dephosphorylation of CheYp. CheYp apparently binds first to the C terminus of CheZ...
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In the chemotaxis pathway of the bacterium Escherichia coli, signals are carried from a cluster of receptors to the flagellar motors by the diffusion of the protein CheY-phosphate (CheYp) through the cytoplasm. A second protein, CheZ, which promotes dephosphorylation of CheYp, partially colocalizes with receptors in the plasma membrane. CheZ is normally dimeric in solution but has been suggeste...
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تاریخ انتشار 2007